mcColocStatsInd Enumeration |
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Namespace: MediaCy.IQL.Features
Assembly: MediaCy.IQL.Features (in MediaCy.IQL.Features.dll) Version: 10.0.6912.0
SyntaxVB
Public Enumeration mcColocStatsInd
Members| Member name | Value | Description | |
|---|---|---|---|
| mcclsiPearson | 0 | Pearson correlation.Pearson’s Correlation is a measure of the linear correlation (dependence) between intensities of two channels, giving a value between +1 and ?1 inclusive, where 1 is total positive correlation, 0 is no correlation, and ?1 is negative correlation. It is a measure of the degree of linear dependence between two channels. This measure uses all pixels, while mcclsi_PearsonAbove uses only colocalized pixels above thresholds | |
| mcclsiOverlapR | 1 | Overlap coefficient.As Pearson’s Correlation does some averaging of pixel information and can return negative values another method, the Overlap Coefficient, is simultaneously used to describe overlap. This method does not perform any pixel averaging functions, so correlations are returned as values between 0 and 1. This method is not sensitive to intensity variations in the image analysis. This is especially important when considering issues such as camera quantum efficiency, hybridization efficiency and sample photobleaching. | |
| mcclsi_k1 | 2 | Overlap coefficient k1.These coefficients describe the differences in intensities of red and green. Simply put, the value k1 is sensitive to differences in intensity for green while k2 is sensitive to differences in intensity for red. | |
| mcclsi_k2 | 3 | Overlap coefficient k2 | |
| mcclsi_m1 | 4 | Colocalization coefficient m1.m1 and m2 estimate the contribution of one color channel in the colocalized areas of the image to the overall colocalized fluorescence in the image. m1 is used to describe the contribution of green to the colocalized area while m2 is used to describe the contribution of red. | |
| mcclsi_m2 | 5 | Colocalization coefficient m2. | |
| mcclsi_Intersection | 6 | Colocalization coefficient Intersection.Ratio between colocalized area and the total area of the object. | |
| mcclsi_i1 | 7 | Colocalization coefficient i1.Ratio between colocalized area and the red area above threshold. | |
| mcclsi_i2 | 8 | Colocalization coefficient i2.Ratio between colocalized area and the green area above threshold. | |
| mcclsi_lM | 9 | M coefficient of regression line of 2 channels scatterplot (line equation is mx+b) | |
| mcclsi_lB | 10 | B coefficient of regression line of 2 channels scatterplot (line equation is mx+b) | |
| mcclsi_PearsonAbove | 11 | Pearson correlation of only colocalized pixels above colocalization thresholds | |
| mcclsi_m1t | 12 | Colocalization coefficient m1t.m1 and m2 estimate the thresholded contribution of one color channel in the colocalized areas of the image to the overall colocalized fluorescence in the image. m1 is used to describe the contribution of green to the colocalized area while m2 is used to describe the contribution of red. | |
| mcclsi_m2t | 13 | Colocalization coefficient m2t. |
See Also