CreateColocImageCommand Class |
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Creates colocalization image.
Inheritance HierarchySystemObject
MediaCy.IQL.ApplicationMcCommand
MediaCy.IQL.ApplicationMcCommandExtended
MediaCy.CommandsBaseCommand
MediaCy.CommandsOneInputOneOutputCommandObject, McImage
MediaCy.CommandsTwoInputsOneOutputCommandObject, Object, McImage
MediaCy.Addins.ColocalizationCreateColocImageCommand
MediaCy.IQL.ApplicationMcCommand
MediaCy.IQL.ApplicationMcCommandExtended
MediaCy.CommandsBaseCommand
MediaCy.CommandsOneInputOneOutputCommandObject, McImage
MediaCy.CommandsTwoInputsOneOutputCommandObject, Object, McImage
MediaCy.Addins.ColocalizationCreateColocImageCommand
Namespace: MediaCy.Addins.Colocalization
Assembly: MediaCy.Addins.Colocalization (in MediaCy.Addins.Colocalization.dll) Version: 3.1.0.0
SyntaxVB
Public Class CreateColocImageCommand Inherits TwoInputsOneOutputCommand(Of Object, Object, McImage)
The CreateColocImageCommand type exposes the following members.
Constructors| Name | Description | |
|---|---|---|
 | CreateColocImageCommand | Initializes a new instance of the CreateColocImageCommand class |
| CreateColocImageCommand(McCommand) | Initializes a new instance of the CreateColocImageCommand class |
Properties| Name | Description | |
|---|---|---|
 | AutoThreshold |
Auto- threshold algorithm, which is used when thresholds are below zero.
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| Channel1 |
First channel for colocalization. The channel index of Input.
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| Channel2 |
Second channel for colocalization. If Input is a mono image and Input2 is defined, then Channel2 points to the second channel in Input2,
in that case it should be 0 since input and Input2 should be monochrome images.
If Input is a multi-channel image, then Channel2 points to the second channel in Input.
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| ChannelName1 |
Channel name 1 (optional)
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| ChannelName2 |
Channel name 2 (optional)
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| Color1 |
Color of the first channel for colocalization, it's used only when ColorPair = eColorPair.Custom
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| Color2 |
Color of the second channel for colocalization, it's used only when ColorPair = eColorPair.Custom
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| ColorPair |
Color pair of colocalization. If it's Custom, then channels are defined by Channel and Color properties
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| Creator |
Creator, used with Protocols
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| ExternalParentChannel |
Property used with MCA when parent channel is defined externally
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| Input3 |
Parent channel image
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| Output2 |
Co-localization statistics
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| Output3 |
Composite image, which is created when 2 input images are provided.
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| ParentChannel |
Index of parent channel (-1 if parent channel is not used)
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| ResultType |
Result type of colocalization.
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| Scale |
Scale of thresholds. Output property, calculated as (RangeMax+1)/256.
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| Threshold1 |
Threshold of channel 1 for calculating colocalization values. If the value is -1, then the threshold is calculated automatically.
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| Threshold2 |
Threshold of channel 2 for calculating colocalization values.
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| Visible |
Output Image visible flag.
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Methods| Name | Description | |
|---|---|---|
| Run |
Runs the CreateColocImageCommand.
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| SetOptions |
Set from options
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RemarksNote, that Visible flag affects only composite image (Output3). The colocalization image is returned as invisible.
See Also